UniWells™ Horizontal Co-Culture Plate
UniWells™ Horizontal Co-Culture Plate is an ideal co-culture device that connects two wells laterally.
Horizontal connection provides easy viewing of cells in both wells simultaneously using various type of microscopes.
It is a versatile tool for studying cell-cell interactions such as transport, migration and invasion.
Features
- Simultaneous observation using a time-lapse microscope
- No more filter clogging
- Cells are cultured under the same condition (used same material on the bottom)
- Filters of any membrane types and pore sizes are usable
- Both wells are independent (connect in a free combination)
Excellent point
Both cells can be cultured under the same medium volume
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Conventional co-colture plate
Medium volume in A is more than in B and cell secreted factors A are diluted in B. -
UniWells™
Medium volume are the same between A and B
Filter is prevented from being clogged by cells
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Conventional co-colture plate
The filter is clogged by cells cultured in the upper well, which leads to interfere with migration of cell secreted factors between the upper and lower wells. -
UniWells™
The filter is NOT clogged by cells.
Kit Contents
UniWells™ Horizontal Co-Culture Plate (384-14421)
| Main Body A | ・・・2 |
| Main Body B | ・・・2 |
| Cover | ・・・4 |
| Common Cover | ・・・4 |
| Adapter | ・・・1 |
| O-ring | ・・・2 |
| [Note] The filters are sold separately | |
Example of use – Uptake of exosomes -
UniWells™ Horizontal Co-Culture Plate enables the observation that exosomes derived from cell B permeate the filter and are uptaked by cell A.
Comparison of permeability testing of exosome between conventional co-culture plate and UniWells™
- Cells were seeded to be the same cell numbers into the upper well in conventional co-culture plate and one side well in Uniwells™.
- Co-culture started after 24 hours from cell seeding.
- Exosome densities of cell side and medium side were analyzed.
Instructions for use
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Connected use
Wells are connected by fitting a filter, O-ring and cover.
※The filters are sold separately.
Connection methods are as below;
A)Connect the wells cultured independently by aspirating the culture solution once.
B)Connect first and increase the volume of the culture solution to achieve co-culture
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Single use
Fit a common cover and cover to a well.
Use in a microscope
Set wells to the adapter provided.
※The adapter is attached to the main unit.
Medium volume
|
Medium volume less than 400 μL is not shared between the wells.  |
The maximum medium volume on each well is 1.8 mL.  |
FAQ
What is UniWells™ made of ?
Main body and common cover are made of polystyrene and low density polyethylene, respectively. UniWells™ filter, which is sold separately, is made of polycarbonate.
Is Uniwells™ Sterilized ?
Uniwells™ is sterilized by electron beam sterilization. Do not sterilize it by autoclaving.
Are the main body surfaces coated with anything ?
No, they are not coated. Coat the main body surfaces with attachment matrixes as needed.