Rapid production of mature neurons

Approximately 1/2 of conventional culture method

Neuron culture medium: 14 days

Conventional culture medium: approximately 1 month

Low density culture

1/5 - 1/10 the number of cells compared with conventional methods

Neuron culture medium: 0.1 ×10⁶ cells/mL

Conventional culture medium: 0.5～1.0×10⁶ cells/mL

Ready-to-Use

Cells can be cultured with the culture medium alone, with no need for additional supplements.

Rat hippocampal neurons isolated from E19 embryos were cultured using poly-L-lysine-coated microtiter plates. 
The morphology of these neurons was analyzed on day 6. We also examined the expression of the neuronal markers MAP2(a+b) and the glial marker GFAP on day 21

Day 6

< Growth Medium >
NS Basal Medium containing 2% NS Supplement and 0.5 mM L-glutamine

< Seeding Density >
13,000cells/well（96well plate）

Day 21

Primary cortical neurons isolated from E17 rat embryos were cultured using poly-L-lysine-coated microtiter plates. 
The morphology of these neurons was analyzed on day 8. We also examined the expression of the neuronal markers MAP2(a+b) and the glial marker GFAP on day 14.

Day 8

< Growth Medium >
NS Basal Medium containing 2% NS Supplement and 0.5 mM L-glutamine

< Seeding Density >
60,000cells/well（96well plate）

Day 14