This protocol is available for download on Owens's lab page: And this is a published protocol on Nature Methods:


Step 1: Human or mouse tissue sections

PFA-fixed and paraffin-embedded tissues. Sections: 5µm. (Can try 10um but it is hard to have all important data and all PLA signals focused in the same plane).


Step 2: Immunofluorescent staining

Deparaffinization and rehydration:

  • Xylene: 2x5min
  • EtOH 100% 2x5min
  • EtOH 95% 2x5min
  • EtOH 70%: 1x5min
  • dH2O: 2x5min

Note: Workstation – tissue tek II.

Search Articles