EV-Save™ Extracellular Vesicle Blocking Reagent

For isolation of extracellular vesicles from cell culture supernatants using Mag Capture™ Exosome Isolation Kit PS (Code No. 299-77603, 293-77601)

1. Add EV-Save™ Extracellular Vesicle Blocking Reagent to be diluted 100- fold into cell culture supernatants and mix it by inverting or tapping.
   In the case of concentrating cell culture supernatants by an ultrafiltration unit, add EV-Save™ Extracellular Vesicle Blocking Reagent into cell culture supernatants before ultrafiltration.
2. Proceed with an experiment to the step of “Elution of extracellular vesicles” described in the instruction of MagCapture™ Exosome Isolation Kit PS.
3. Add EV-Save™ Extracellular Vesicle Blocking Reagent to be diluted 100- fold into “Exosome Elution Buffer” accompanied with Mag Capture™ Exosome Isolation Kit PS and mix it by inverting or tapping.
4. Elute extracellular vesicles according to the instruction of Mag Capture™ Exosome Isolation Kit PS.
5. Store the eluted extracellular vesicles, or proceed to the next experiment.

For isolation of extracellular vesicles from cell culture supernatants using other methods

1. Add EV-Save™ Extracellular Vesicle Blocking Reagent to be diluted 100- fold into cell culture supernatants and mix it by inverting or tapping.
2. Add EV-Save™ Extracellular Vesicle Blocking Reagent to be diluted 100-fold into isolated extracellular vesicles and mix it by inverting or tapping.
3. Store it or proceed to the next experiment