This kit is based on binding to the mouse Fc region. If your sample includes human serum, it may interfere with the assay and create a false positive signal. In this instance, we suggest using the SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit, BPS Bioscience #79945.
Coronavirus disease 2019 (COVID-19) increases the risk of developing Acute Respiratory Distress Syndrome (ARDS), which is often fatal at the late stages of the infection when the SAR-CoV-2 virus causes significant damage to the lungs. As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.
The ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of Fc-tagged Spike protein by HRP-labeled Anti-mouse-Fc. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a nickel-coated 96-well plate. Next, SARS-CoV-2 Spike-Fc is incubated with ACE2 on the plate. Finally, the plate is treated with Anti-mouse-Fc-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.
PBS (Phosphate buffered saline)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
This kit is useful for screening for inhibitors of ACE2 binding to SARS-CoV-2 Spike.
Up to 6 months from date of receipt, when stored as recommended.
| Catalog Number |
Component |
Amount |
Storage |
|
| 79932 | SARS-CoV-2 Spike Protein (RBD), mFc Tag | 5 μg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 11003 | ACE2, His-Tag | 5 μg | -80°C | |
| 79311 | 3x Immuno Buffer 1 | 50 ml | -20°C | |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C | |
| 52130H | Secondary HRP-labeled antibody 1 (mouse) | 15 μl | -80°C | |
| 79670 | ELISA ECL substrate A (transparent bottle) | 6 ml | Room Temp |
|
| ELISA ECL substrate B (brown bottle) | 6 ml | Room Temp |
||
| Nickel-coated 96-well white microplate | 1 | +4°C | ||
See assay kit protocol for detailed instructions.
Hoffmann, M. et al. 2020. Cell, 181(2):271-280.e1-e5
Yan, R. et al. 2020. Science, 367(6485):1444-1448
Application Reference(s):
Avoid multiple freez/thaw cycles.