Principle: mini spin column (silica matrix)

Sample size: 1 ~ 5 ml br> 

Size of plasmid or construct: 15 kb br> 

Operation time: 25 minutes

Typical Yield: 20 ~ 30 µg

Binding capacity: 60 µg/ column

Column applicability: centrifugation and vaccum

Important Notes:

1. Store RNase A at -20 °C upon recipit of kit.

2. Add 0.5 ml of FAPD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the FAPD1 bottle, mix well by vortexing and store the FAPD1 buffer at 4 °C.

3. If precipitates have formed in FAPD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.

4. Preparation of W1 Buffer and Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.

5. Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.

◆Store at room temperature (15~ 25 ℃) for 2 year. Except RNAse A Powder, store at -20℃.

Principle: mini spin column (silica matrix)

Sample size: 1 ~ 7 ml 

Size of plasmid or construct: <15 kb 

Operation time: <25 minutes

Typical Yield: 25 ~ 40 µg

Binding capacity: 60 µg/ column

Column applicability: centrifugation and vaccum

Important Notes:

1. Store RNase A at -20 °C upon recipit of kit.

2. Add 0.5 ml of FAPD1 Buffer to a RNase A tube, Dissolve the RNase A by vortexing. Briefly spin the tube and transfer the total RNase A mixture back to the FAPD1 bottle, mix well by vortexing and store the FAPD1 buffer at 4 °C.

3. If precipitates have formed in FAPD2 Buffer, warm the buffer in 37°C waterbath to dissolve precipitates.

4. Preparation of W1 Buffer and Wash Buffer by adding 96 ~100% ethanol (not provided) for first use.

5. Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.

◆Store at room temperature (15~ 25 ℃) for 2 year. Except RNAse A Powder, store at -20℃.