We are committed to provide complete solutions

for life science applications enabling people

to make and deliver life improving discoveries.

ACE2:SARS-CoV-2 Spike S1-Biotin Inhibitor Screening Assay Kit

PDFPrintEmail
  • Product Code: #79945
  • Packaging: 96 reactions
  • Base price 13005 元
    Sales price 13005 元
    Discount
    Price / kg:
    Description
    Description

    Coronavirus disease 2019 (COVID-19) increases the risk of developing Acute Respiratory Distress Syndrome (ARDS), which is often fatal at the late stages of the infection when the SAR-CoV-2 virus causes significant damage to the lungs. As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike S1 protein recognizes and attaches to the Angiotensin Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike S1 protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.

    The ACE2:SARS-CoV-2 Spike S1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of Spike S1-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a nickel-coated 96-well plate. Next, SARS-CoV-2 Spike S1-Biotin is incubated with ACE2 on the plate. Finally, the plate is treated with Streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.

    Materials Required But Not Supplied

    PBS (Phosphate buffered saline)
    Luminometer or fluorescent microplate reader capable of reading chemiluminescence
    Adjustable micropipettor and sterile tips

     
    Applications

    This kit is useful for screening for inhibitors of ACE2 binding to SARS-CoV-2 Spike S1.

     
    Supplied As
    This kit comes in a convenient 96-well format, with purified ACE2 and SARS-CoV-2 Spike S1 proteins, Streptavidin-HRP, and assay buffer for 100 binding reactions.
     
    Storage/Stability

    Up to 6 months from date of receipt, when stored as recommended.

     
    Synonym(s)
    Coronavirus, nCoV-2019, nCoV2019, ACEH Screening kit, SARS spike ELISA, ACEH ELISA, covid, covid-19, covid19, Sars-cov-2, sars cov 2, sarscov2
     
    Format

     

    Catalog
    Number

    Component

    Amount

    Storage
    100679 SARS-CoV-2 Spike S1, Fc Fusion, Avi-tag, Biotin-labeled 10 µg -80°C






    Avoid
    multiple
    freeze/
    thaw
    cycles
    11003 ACE2, His-Tag 5 µg -80°C
    79311 3x Immuno Buffer 1 50 ml -20°C
    79728 Blocking Buffer 2 50 ml +4°C
    79742 Streptavidin-HRP 15 µl -20°C



    79670
    ELISA ECL substrate A (transparent bottle) 6 ml +4°C
    ELISA ECL substrate B
    (brown bottle)
    6 ml +4°C
      Nickel-coated 96-well white microplate 1 +4°C
     
    Instructions for Use

    See assay protocl for setailed instructions.

     
    References

    Hoffmann, M. et al. 2020. Cell 181:1-10
    Yan, R. et al. 2020. Science 367(6485): 1444-1448.

     
    Warnings

    Avoid freeze/thaw cycles

     
    Scientific Category
    Coronavirus

    Reviews

    There are yet no reviews for this product.

    We are committed to provide complete solutions

    for life science applications enabling people

    to make and deliver life improving discoveries.