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ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit

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  • Product Code: #79936
  • Packaging: 96 rxns.
  • Base price 10200 元
    Sales price 10200 元
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    Description
    Description

    This kit is based on binding to the mouse Fc region. If your sample includes human serum, it may interfere with the assay and create a false positive signal. In this instance, we suggest using the SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit, BPS Bioscience #79945.

    Coronavirus disease 2019 (COVID-19) increases the risk of developing Acute Respiratory Distress Syndrome (ARDS), which is often fatal at the late stages of the infection when the SAR-CoV-2 virus causes significant damage to the lungs. As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer some protection against the viral infection.



    The ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. The key to this kit is the high sensitivity of detection of Fc-tagged Spike protein by HRP-labeled Anti-mouse-Fc. Only a few simple steps on a microtiter plate are required for the assay. First, ACE2 protein is attached to a nickel-coated 96-well plate. Next, SARS-CoV-2 Spike-Fc is incubated with ACE2 on the plate. Finally, the plate is treated with Anti-mouse-Fc-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.

    Materials Required But Not Supplied

    PBS (Phosphate buffered saline)
    Luminometer or fluorescent microplate reader capable of reading chemiluminescence
    Adjustable micropipettor and sterile tips

     
    Applications

    This kit is useful for screening for inhibitors of ACE2 binding to SARS-CoV-2 Spike.

     
    Supplied As
    This kit comes in a convenient 96-well format, with purified ACE2 and SARS-CoV-2 Spike proteins, HRP-labeled anti-mouse Fc region antibody, and assay buffer for 100 binding reactions.
     
    Storage/Stability

    Up to 6 months from date of receipt, when stored as recommended.

     
    Synonym(s)
    ACEH, covid, covid-19, covid19, and coronavirus
     
    Format
    Catalog
    Number

    Component

    Amount

    Storage
    79932 SARS-CoV-2 Spike Protein (RBD), mFc Tag  5 μg  -80°C




    Avoid
    multiple
    freeze/
    thaw
    cycles!       
    11003  ACE2, His-Tag  5 μg  -80°C
    79311 3x Immuno Buffer 1  50 ml  -20°C
    79728 Blocking Buffer 2  50 ml  +4°C
    52130H Secondary HRP-labeled antibody 1 (mouse) 15 μl  -80°C
    79670 ELISA ECL substrate A (transparent bottle)  6 ml Room
    Temp
    ELISA ECL substrate B (brown bottle)  6 ml  Room
    Temp
      Nickel-coated 96-well white microplate  1  +4°C
     
    Instructions for Use

    See assay kit protocol for detailed instructions.

     
    References

    Hoffmann, M. et al. 2020. Cell181(2):271-280.e1-e5
    Yan, R. et al. 2020. Science367(6485):1444-1448

    Application Reference(s):

    1. Hijacking SARS-Cov-2/ACE2 receptor interaction by natural and semi-synthetic steroidal agents acting on functional pockets on receptor binding region (2020)

     
    Warnings

    Avoid multiple freez/thaw cycles.

     
    Scientific Category
    Coronavirus
     

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    We are committed to provide complete solutions

    for life science applications enabling people

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